| Popis: |
Objective: This present study aimed to establish collagen-induced arthritis (CIA) osteoporosis mice model and dynamically observe the pathological and immune characteristics of CIA osteoporosis mice. Methods: Bovine collagen type was chosen to build the CIA model. The corresponding indexes were observed at 6w, 10w, 14w and 18w after successful modelling: Arthritis Index were used to evaluate RA. The structure and pathological changes of bone and joint were observed by pathological section. X ray film was used to observe morphology of bone and joint; And the serum IL-17 and osteoprotegerin (OPG) levels were detected by Elisa. Rt-PCR was used to assess the mRNA levels of IL-17, NFAT, ROR-γt and Foxp3 in spleen, the mRNA levels of OPG and RANK in bone tissue. Results: In the model group at 6w after modelling, AI and pedal swelling volume began to be increased gradually. Compared with the normal group, the serum IL-17 level in the model group increased gradually with time, the serum OPG level was considerably decreased, the mRNA levels of IL-17, NFAT, ROR- γt, etc. in spleen tissue increased. However, the level of Foxp3 mRNA in the spleen of model group reduced. The expression of OPG mRNA in bone tissues was dropped. RANK mRNA expression was increased. Bone density of both femur and tibia were decreased gradually with time, leading to osteoporosis. X-ray transmittance of the spine increased with time, and the spine presented water-wavy changes at 14w. Conclusion: RA mice model successfully induced by bovine collagen type Ⅱ, and the extension of lesions aggravated over time; Th17 cells results RANKL / OPG and Treg / TH17 imbalance by excessive secreting IL-17, leading to activation of osteoclasts and inhibit the expression of OPG. Therefore, it was involved in the occurrence of osteoporosis in RA in mice. |
