Real-time monitoring DNA hybridization by guided resonant mode biosensor
| Autor: | Ting-Jou Ding, Wen-Yih Chen, Jenq Yang Chang, Tsung Hsun Yang, Jen-Tsai Liu, Hsin-Chun Huang, Che-Lung Hsu, Jiann-Hwa Lue |
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| Rok vydání: | 2011 |
| Předmět: |
animal structures
Materials science Guided-mode resonance business.industry DNA–DNA hybridization fungi Analytical chemistry Lab-on-a-chip law.invention body regions chemistry.chemical_compound Nucleic acid thermodynamics chemistry law Optoelectronics DNA microarray business Spectroscopy Biosensor DNA |
| Zdroj: | 2011 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. |
| DOI: | 10.1109/nems.2011.6017574 |
| Popis: | In this paper, a label free approach of the DNA hybridization detection by the guided-mode resonance (GMR) spectroscopy sensor is presented. The GMR biosensor can easily detect any slight variation of the DNA hybridization by the resonant wavelength peak shift. The corresponding peak wavelength shifts of the capture DNA, the target DNA and the probe DNA serially hybridized reactions from GMR are measured, respectively. Furthermore, the stability and reliability of the GMR biosensor for nucleic acid hybridization have also been examined. The result obviously reveals the feasibility of implementation of the GMR-based DNA microarray for detecting on the dynamics of DNA hybridization. |
| Databáze: | OpenAIRE |
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