Fractionation of cell constituents ofBacillus megaterium in a polymer two-phase system
| Autor: | Bengt v. Hofsten, G. David Baird |
|---|---|
| Rok vydání: | 1962 |
| Předmět: |
Aqueous solution
Chromatography biology Sodium fungi food and beverages chemistry.chemical_element Bioengineering Fractionation biology.organism_classification Applied Microbiology and Biotechnology Suspension (chemistry) Cell wall chemistry.chemical_compound Dextran chemistry Phase (matter) Biotechnology Bacillus megaterium |
| Zdroj: | Biotechnology and Bioengineering. 4:403-410 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.260040406 |
| Popis: | The distribution of various cell constituents of Bacillus megaterium has been studied in an aqueous two-phase system containing the two polymers dextran and polythylene glycol. The difference in behaviour between cell walls, lipid granules and soluble cell material are such that a considerable fractionation of a suspension of disrupted cells may be obtained after a few counter-current distributions. A mixture of whole cells and protoplasts can also be fractionated in this phase system. If sodium chloride is added to the phase system, vegetative cells and spores can be separated from each other. |
| Databáze: | OpenAIRE |
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