| Autor: |
Katja Tönsing, Peter Reimann, Andre Spiering, L. Redondo, Sebastian Knust, André Beyer, Sebastian Getfert, Karsten Rott, Dario Anselmetti, Andy Sischka |
| Rok vydání: |
2012 |
| Předmět: |
|
| DOI: |
10.1039/9781849735278-00099 |
| Popis: |
The controlled translocation of a single, double-stranded DNA (dsDNA) through a solid-state nanopore (NP) with optical tweezers (OT) is described in the presence of an electric field under buffer conditions. Upon threading dsDNA complexed by single proteins through a NP in 20 nm thick Si3N4-membranes, we find distinct asymmetric and retarded force signals that critically depend on the overall charge of the protein, the molecular elasticity of the dsDNA and the counter-ionic shielding of the polyelectrolyte (dsDNA) in the buffer3. This force response can be quantitatively simulated and understood within a theoretical model where an isolated charge on an elastic, polyelectrolyte strand experiences a harmonic nanopore potential during translocation. In order to extend these experiments to atomically thin solid-state NP, dsDNA was threaded through single nanolayer graphene NP by a transmembrane voltage. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
