571. Development of a Quantitative Potency Assay for Quality Control Testing for pVGI.1(VEGF2), a Naked DNA Vector

Autor:	Jiemin Zhou, Li-chun Huang, Hsing-yi Jan, Yawen L. Chiang, Qing Wang
Rok vydání:	2005
Předmět:	Pharmacology Base pair Angiogenesis Chemotaxis Biology Molecular biology In vitro Plasmid Naked DNA Drug Discovery Genetics Molecular Medicine Potency Molecular Biology Gene
Zdroj:	Molecular Therapy. 11:S220
ISSN:	1525-0016
DOI:	10.1016/j.ymthe.2005.07.111
Popis:	The plasmid pVGI.1(VEGF2) contains 5283 base pairs and encodes the full length cDNA of the VEGF-2 gene. Preclinical studies and earlier clinical trials demonstrated that injecting the pVGI.1(VEGF2) directly into muscles provided therapeutic angiogenic effects both in the heart (CAD) and in the limb (PAD). Presumably, the VEGF-2 protein expressed from pVGI.1(VEGF2) promotes angiogenesis in the injected tissues. In vitro, it has been shown that the purified VEGF-2 protein mediated proliferative and chemotactic responses in endothelial cells through the binding to the VEGF receptors VEGFR-2 (KDR) or VEGFR-3 (Flt-4).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::07aa29c4a5110fc2566c9fac65fba53c https://doi.org/10.1016/j.ymthe.2005.07.111 Zobrazit plný text záznamu