| Autor: |
Charles Danko, Gilad Barshad, James Lewis, Alexandra Chivu, Abderhman Abuhashem, Nils Krietenstein, Edward Rice, Oliver J. Rando, Anna-Katerina Hadjantonakis |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-1851612/v1 |
| Popis: |
How enhancers control target gene expression over long genomic distances remains an important unsolved problem. Here we studied enhancer-promoter contact architecture and communication by integrating data from nucleosome-resolution genomic contact maps, nascent transcription, and perturbations to transcription-associated proteins and thousands of candidate enhancers. Contact frequency between functionally validated enhancer-promoter pairs was most enriched near the +1 and +2 nucleosomes at enhancers and target promoters, indicating that functional enhancer-promoter pairs spend time in close physical proximity. Blocking RNA polymerase II (Pol II) caused major disruptions to enhancer-promoter contacts. Paused Pol II occupancy and the enzymatic activity of poly (ADP-ribose) polymerase 1 (PARP1) stabilized enhancer-promoter contacts. Based on our findings, we propose an updated model that couples transcriptional dynamics and enhancer-promoter communication. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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