| Popis: |
Publisher Summary The transport of proteins between the different organelles or compartments that make up the secretory and endocytic pathways is thought to be mediated by small vesicles that bud off from one compartment and subsequently fuse with their target compartment. Three steps in the secretory pathway are reconstituted to date, which are, transport from the endoplasmic reticulum (ER) to the Golgi compartment, transport from the cis- to the medial-Golgi compartment, and transport from the medial- to the trans-Golgi compartment. The cell-free system used to reconstitute cis- to medial-Golgi transport provides with most of the information to date regarding the biochemical pathway of vesicular transport and the specific cofactors involved. In this system, transport of the vesicular stomatitis virus glycoprorein (VSVG protein) is detected between the cis compartment of a mutant Golgi preparation (donor membranes) and the medial compartment of a wild-type Golgi preparation. This system has contributed in a significant way to the understanding of vesicular transport. Not only has it made possible the identification and purification of a number of proteins that are required for transport, it has also made possible the identification, for the first time, of a number of possible reaction intermediates in vesicular transport. |
