Cell type‐specific distribution of GABA A receptor subtypes in the mouse dorsal striatum
| Autor: | Jean-Marc Fritschy, Leonardo Cristiá-Lara, Lena Rubi, Ida Luisa Boccalaro, Cornelia Schwerdel |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
education.field_of_study animal structures Interneuron GABAA receptor musculoskeletal neural and ocular physiology General Neuroscience Population Striatum Biology Medium spiny neuron 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure nervous system Postsynaptic potential medicine Cholinergic GABAergic sense organs education Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Comparative Neurology. 527:2030-2046 |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.24665 |
| Popis: | The striatum is the main input nucleus of the basal ganglia, mediating motor and cognitive functions. Striatal projection neurons are GABAergic medium spiny neurons (MSN), expressing either the dopamine receptor type 1 (D1 -R MSN) and forming the direct, movement-promoting pathway, or dopamine receptor type 2 (D2 -R MSN), forming the indirect movement-suppressing pathway. Locally, activity and synchronization of MSN are modulated by several subtypes of GABAergic and cholinergic interneurons. Overall, GABAergic circuits in the striatum remain poorly characterized, and little is known about the intrastriatal connectivity of interneurons and the distribution of GABAA receptor (GABAA R) subtypes, distinguished by their subunit composition, in striatal synapses. Here, by using immunofluorescence in mouse tissue, we investigated the distribution of GABAA Rs containing the α1 , α2 , or α3 subunit in perisomatic synapses of striatal MSN and interneurons, as well as the innervation pattern of D1 R- and D2 R-MSN soma and axonal initial segment (AIS) by GABAergic and cholinergic interneurons. Our results show that perisomatic GABAergic synapses of D1 R- and D2 R-MSN contain the GABAA R α1 and/or α2 subunits, but not the α3 subunit; D2 R-MSN have significantly more α1 -GABAA Rs on their soma than D1 R-MSN. Further, interneurons have few perisomatic synapses containing α2 -GABAA Rs, whereas α3 -GABAA Rs (along with the α1 -GABAA Rs) are abundant in perisomatic synapses of CCK+ , NPY+ /SOM+ , and vAChT+ interneurons. Each MSN and interneuron population analyzed received a distinct pattern of GABAergic and cholinergic innervation, complementing this postsynaptic heterogeneity. In conclusion, intra-striatal GABAergic circuits are distinguished by cell-type specific innervation patterns, differential expression and postsynaptic targeting of GABAA R subtypes. |
| Databáze: | OpenAIRE |
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