Effects of riboflavin and AQS as electron shuttles on U(vi ) reduction and precipitation byShewanella putrefaciens
| Autor: | Mingxue Liu, Tingting Huo, Pingping Wang, Wang Xuhui, Hongfu Wei, Lei Zhou, Faqin Dong, Wei Zhang, Xiaoqin Nie |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
biology
Precipitation (chemistry) Chemistry General Chemical Engineering 02 engineering and technology General Chemistry Flavin group 010501 environmental sciences Shewanella putrefaciens 021001 nanoscience & nanotechnology Electrochemistry biology.organism_classification 01 natural sciences Electron transfer Bioremediation X-ray photoelectron spectroscopy Fourier transform infrared spectroscopy 0210 nano-technology 0105 earth and related environmental sciences Nuclear chemistry |
| Zdroj: | RSC Advances. 8:30692-30700 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c8ra05715j |
| Popis: | Understanding the mechanisms for electron shuttles (ESs) in microbial extracellular electron transfer (EET) is important in biogeochemical cycles, bioremediation applications, as well as bioenergy strategies. In this work, we adapted electrochemical techniques to probe electrochemically active and redox-active Shewanella putrefaciens. This approach detected flavins and humic-like substances of Shewanella putrefaciens, which were involved in electron transfer to the electrode. A combination of three-dimensional excitation-emission (EEM) florescence spectroscopy methods identified a mixture of riboflavin and humic-like substances in supernatants during sustained incubations. The reductive behaviour of U(VI) by Shewanella putrefaciens in the presence of riboflavin (RF) and anthraquinone-2-sulfonate (AQS) was also investigated in this study. The results indicated that RF and AQS significantly accelerated electron transfer from cells to U(VI), thus enhancing reductive U(VI). The precipitate was further evidenced by SEM, FTIR, XPS and XRD, which demonstrated that chernikovite [H2(UO2)2(PO4)2·8H2O] became the main product on the cell surface of S. putrefaciens. In a contrast, U(IV) mainly existed amorphously on the cell surface of S. putrefaciens with added RF and AQS. This work has significant implications in elucidating RF and AQS as electron shuttles that are efficient in reduction of uranium in geological environments. |
| Databáze: | OpenAIRE |
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