ENZYME ACTIVITIES IN CHICK EMBRYONIC CARTILAGE

Autor:	Reuben Eisenstein, Klaus E. Kuettner, L. W. Soble, C. Arsenis
Rok vydání:	1971
Předmět:	chemistry.chemical_classification Cartilage Sonication Embryo Cell Biology Biology chemistry.chemical_compound medicine.anatomical_structure Enzyme Biochemistry chemistry Extracellular medicine Microsome Cell fractionation Lysozyme
Zdroj:	Journal of Cell Biology. 49:459-467
ISSN:	1540-8140 0021-9525
DOI:	10.1083/jcb.49.2.459
Popis:	Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fractions are characterized by enzymatic activities usually associated with similar fractions in other tissues, but with some quantitative differences. Lysozyme, a particulate-associated enzyme in other tissues, was not detected in any subcellular fraction even by the sensitive technique of microzone electrophoresis and is therefore considered to be primarily extracellular in cartilage.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::079a2af77a7c8f51a45d79877f55640c https://doi.org/10.1083/jcb.49.2.459 Zobrazit plný text záznamu