Redox-active lipid-incorporating proteins as a novel immobilisation technique

Autor:	Claus Jacob, Sonia Wilson, Timothy F. Booth, Alexander Y. Safronov, Stephen K Chapman, H. Allen O. Hill
Rok vydání:	1997
Předmět:	Chemistry Stereochemistry General Chemical Engineering Bilayer Enzyme electrode Analytical Chemistry Ion selective electrode Electron transfer chemistry.chemical_compound Cytochrome b2 Crystallography Ferrocene Electrode Amphiphile Electrochemistry
Zdroj:	Journal of Electroanalytical Chemistry. 430:119-125
ISSN:	1572-6657
DOI:	10.1016/s0022-0728(97)00102-2
Popis:	A novel chiral redox-active ferrocene compound (FcV1) with amphiphilic properties has been synthesised and used successfully to form protein + lipid ‘clusters’ with ferritin and gold-labelled BSA. These structures could be observed by cryogenic transmission electron microscopy and had diameters between 40 and 300 nm. It was shown that the novel lipid formed reasonably stable films on an edge plane graphite electrode surface and also mediated the electron transfer between flavocytochrome b 2 and this electrode. Consequently, the enzyme was incorporated into an FcV1 film and its response towards l (+)lactate in solution investigated. It could be shown that the electrode indeed was sensitive to l (+)lactate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0793ad6c8e2f070e3121630bd22742cb https://doi.org/10.1016/s0022-0728(97)00102-2 Zobrazit plný text záznamu Full Text from ScienceDirect