Characterization of Salmonella and possible interfering strains using GC profiling and factor analysis
| Autor: | G. Wieten, J. de Waart, Albert C. Tas, J. van der Greef, L. Berwald |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
Chemical ionization Salmonella Chromatography biology Chemistry biology.organism_classification Mass spectrometry medicine.disease_cause Microbiology Enterobacteriaceae chemistry.chemical_compound medicine Gas chromatography Diglyceride Derivatization Molecular Biology Fatty acid methyl ester |
| Zdroj: | Journal of Microbiological Methods. 8:333-345 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/0167-7012(88)90032-2 |
| Popis: | Fatty acid methyl ester data of Salmonella and potentially interfering strains obtained by derivatization gas chromatography were investigated with principal components and factor-discriminant analysis. Salmonella strains could be distinguished using both data-handling techniques. The biochemical basis for this characterization was found to be in strong agreement with the higher mass range part of pyrolysis — direct chemical ionization mass spectrometric data representing diglyceride information. The quality of the analytical procedure as judged by longer term reproducibility and component assignment was found to be good. In combination with sophisticated data-handling methods this derivatization gas chromatographic approach can be a useful diagnostic tool for Salmonella characterization. |
| Databáze: | OpenAIRE |
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