Electrochemical Dissolved Oxygen Sensor-Integrated Platform for Wireless In Situ Bioprocess Monitoring
| Autor: | Reza Ghodssi, Justin M. Stine, William E. Bentley, Luke A. Beardslee, Rajendra Mayavan Sathyam |
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| Rok vydání: | 2020 |
| Předmět: |
Detection limit
Materials science business.industry Metals and Alloys 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Potentiostat 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Electrochemical gas sensor Microcontroller Electrode Materials Chemistry Bioreactor Electrical and Electronic Engineering Bioprocess 0210 nano-technology Process engineering business Saturation (chemistry) Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 320:128381 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2020.128381 |
| Popis: | This work presents a bio-processing online device (bPod) platform, capable of real-time in situ monitoring of bioreactor cell culture parameters, such as dissolved oxygen (DO). The bPod is an integrated system comprised of a potentiostat analog-front-end (AFE), a Bluetooth Low Energy (BLE) microcontroller, and a chemical sensor, exemplified here by a Clark-type DO sensor, which enables monitoring of dissolved oxygen content. The Clark-type electrochemical sensor performs chronoamperometric measurement of DO percent saturation, and the BLE microcontroller wirelessly transmits data to a smartphone while submerged in aqueous media. After several electrode design modifications, the bPod showed a linear electrochemical current response corresponding to DO percent saturation levels with a sensitivity of 37.5 nA/DO% and limit of detection of 8.26 DO%, covering concentration ranges relevant for mammalian culture processes within bioreactors. The wireless bPod provides a free-floating capsule architecture for monitoring DO and can be adapted for an array of electrochemical sensors, targeting different process parameters for diverse bioprocess monitoring applications. |
| Databáze: | OpenAIRE |
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