Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl
| Autor: | Roni Roy, A F M Shahid Ud Daula, Ummah Hafsa Mukta, Israt Jahan Liya, Sohel Mia, Afroza Akter, Mohammad Anwarul Basher, Anwesha Chowdhury, Mahmuda Ferdous |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Antioxidant biology Traditional medicine Chemistry medicine.drug_class DPPH medicine.medical_treatment biology.organism_classification Ascorbic acid 01 natural sciences Lycopene 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Flavonols Phytochemical 010608 biotechnology Drug Discovery Antidiarrhoeal medicine Fimbristylis miliacea |
| Zdroj: | Journal of Pharmacognosy and Phytotherapy. 12:10-18 |
| ISSN: | 2141-2502 |
| DOI: | 10.5897/jpp2019.0567 |
| Popis: | The present study determined phytochemical content, antioxidant and antidiarrhoeal effect of methanol extract of Fimbristylis miliacea. Phytochemical screening showed the presence of flavonoids, saponins, tannins, phenols, alkaloids and cardiac glycosides. Total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found 154.13 mg GAE/g, 83.14 mg QE/g, 126.45 mg QE/g, 215.72 mg GAE/g, 1.51 µg/g, 0.66 µg/g, 7.10 mg/g and 4.25 mg/g, respectively. Antioxidant potential was compared against ascorbic acid. IC50 and EC50 values were determined as indices of antioxidant potential using five experiments; IC50 in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assay were 138.83 and 353.82 µg/ml respectively. EC50 of thiobarbituric acid assay, FRAP assay and reducing power assay were observed 2159.5, 31.59 and 129.56 µg/ml respectively. Total antioxidant capacity was 155.35 mg/g AAE. Significant reduction (p≤0.001) in fecal movement was produced by plant extract and the effect was stronger than that of the standard drug loperamide (5 mg/kg.bw). Loperamide showed 58.16% inhibition whereas extract at 400 mg/kg.bw dose inhibited 80.85% diarrhea. Key words: Fimbristylis miliacea, phytochemical content, antioxidant, antidiarrhoeal |
| Databáze: | OpenAIRE |
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