| Autor: |
R. Rao Gadde, James E. DiNunzio |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Journal of Chromatography A. 594:209-216 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/0021-9673(92)80332-o |
| Popis: |
This paper reports on the development of high-performance liquid chromatographic methods for the determination of hydroxamic acids in topical formulations. A poly(styrene-divinylbenzene)copolymer stationary phase with a mobile phase of acetonitrile and pH 6 phosphate buffer at a flow-rate of 1.0 ml/min was used. Analyte detection was at 254 nm. This system is capable of the separation of phenylalkylhydroxamic acids and benzohydroxamic acids. Excellent quantitative results were obtained. The recoveries from both cream and gel formulations were > 99% with The selectivity of the method using the polymeric stationary phase was investigated. Variation of the mobile phase composition and pH showed that the selectivity of the method could be easily changed by changing mobile phase pH. The method selectivity was demonstrated by forced degradation of selected acids and separation of the analyte from the products. Decomposition to the corresponding carboxylic acid was found to be a major pathway for degradation of these compounds. The separation of the hydroxamic acids on silica bonded phases was also investigated. It was lound that the poor chromatography observed on these phases resulted from the presence of metal impurities in the silica support. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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