Inhibition of Interleukin 1-lnduced Biosynthesis of Stromelysin by the Calcium Antagonist TMB-(8-(N, N-Diethylamino)octyl-3,4,5-trimethoxybenzoate HCl)
| Autor: | Vijaykumar M. Baragi, Wendy S. Rosebury, Ann Marie Sweet, Kathleen L. Hawkins, Mark A. Thompson, Katherine A. Toy |
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| Rok vydání: | 1995 |
| Předmět: |
Chemistry
Immunoprecipitation Antagonist Interleukin chemistry.chemical_element Cell Biology Matrix metalloproteinase Calcium Biochemistry Calcium in biology chemistry.chemical_compound medicine.anatomical_structure Rheumatology Biosynthesis medicine Orthopedics and Sports Medicine Fibroblast Molecular Biology |
| Zdroj: | Connective Tissue Research. 31:153-160 |
| ISSN: | 1607-8438 0300-8207 |
| Popis: | This study was done to identify agents that can inhibit interleukin 1 (IL1)-induced stromelysin biosynthesis and to gain insight into the mechanism of IL1 action. For this purpose, various agents known to modulate calcium-dependent signal transduction pathway were evaluated in rabbit synovial fibroblast (RSF) cultures. Only the conditioned medium from RSF treated with the intracellular calcium antagonist TMB-8 (8-(N, N-diethylamino)-octyl 3,4,5-trimethoxybenzoate hydrochloride) had significantly lower proteoglycan-degrading metalloproteinase activity than controls. Biosynthetic labeling, immunoprecipitation and immunohistochemical studies, using a polyclonal antibody against rabbit stromelysin, demonstrated that TMB-8 inhibited synthesis stromelysin, the proteoglycan-degrading matrix metalloproteinase. Further evaluation of the TMB-8 effect revealed that the compound had no effect on secretion and that it was not acting by preventing activation of the proenzyme or by inhibiting the enzyme activity. These ... |
| Databáze: | OpenAIRE |
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