Determination of 4'-deoxy-4'-(epimethylamino)avermectin B1 benzoate (MK 0244) and its delta 8,9-isomer in celery and lettuce by HPLC with fluorescence detection
Autor: | Teresa A. Wehner, Patricia C. Tway, Richard S. Egan, Sunil V. Prabhu |
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Rok vydání: | 1991 |
Předmět: |
Chromatography
Extraction (chemistry) Ethyl acetate General Chemistry High-performance liquid chromatography Fluorescence spectroscopy Matrix (chemical analysis) chemistry.chemical_compound chemistry Reagent Botany Trifluoroacetic anhydride General Agricultural and Biological Sciences Derivatization |
Zdroj: | Journal of Agricultural and Food Chemistry. 39:2226-2230 |
ISSN: | 1520-5118 0021-8561 |
DOI: | 10.1021/jf00012a025 |
Popis: | An analytical method has been developed for the extraction, derivatization, and fluorescence HPLC determination of MK-0244 [4"-deoxy-4"-(epimethylamino)avermectin B1 benzoate salt] and its delta 8,9-isomer from lettuce and celery at trace (parts per billion) levels. MK-0244 and its delta 8,9-isomer are extracted with methanol, and the extract is cleaned up on a reverse-phase C8 cartridge followed by a liquid-liquid extraction with ethyl acetate before passing through a propyl sulfonyl cation-exchange cartridge. MK-0244 and its delta 8,9-isomer are then derivatized with trifluoroacetic anhydride/1-methylimidazole/acetonitrile reagent system to form a fluorescent derivative which is separated on a reverse-phase HPLC system and detected with a fluorescence detector. The average recoveries were 95 +/- 10% for celery and 96 +/- 8% for lettuce. For both lettuce and celery matrix, concentrations as low as 2 ppb (S/N = 10) can be detected in a 10-g sample. This method was used to determine the MK-0244 levels in samples from a lettuce field trial. |
Databáze: | OpenAIRE |
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