Factors affecting the amount of genomic DNA extracted from ape faeces and the identification of an improved sample storage method

Autor:	Linda Vigilant, Christophe Boesch, Phillip A. Morin, Anthony M. Nsubuga, Martha M. Robbins, Amy D. Roeder
Rok vydání:	2004
Předmět:	Silica gel Sample (material) Biology Genetic analysis Molecular biology chemistry.chemical_compound genomic DNA Real-time polymerase chain reaction chemistry Genetics Food science Desiccation Ecology Evolution Behavior and Systematics Feces DNA
Zdroj:	Molecular Ecology. 13:2089-2094
ISSN:	1365-294X 0962-1083
Popis:	Genetic analysis using noninvasively collected samples such as faeces continues to pose a formidable challenge because of unpredictable variation in the extent to which usable DNA is obtained. We investigated the influence of multiple variables on the quantity of DNA extracted from faecal samples from wild mountain gorillas and chimpanzees. There was a small negative correlation between temperature at time of collection and the amount of DNA obtained. Storage of samples either in RNAlater solution or dried using silica gel beads produced similar results, but significantly higher amounts of DNA were obtained using a novel protocol that combines a short period of storage in ethanol with subsequent desiccation using silica.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::073580117566c77a545c8944f581a43c https://doi.org/10.1111/j.1365-294x.2004.02207.x Zobrazit plný text záznamu Plný text