Enhanced Metabolism and Knockdown Resistance in a Field Versus a Laboratory Strain of the Soybean Looper (Lepidoptera: Noctuidae)
| Autor: | Jerry B. Graves, Randy L. Rose, B. Roger Leonard, Thomas C. Sparks |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Ecology biology Knockdown resistance General Medicine Metabolism Monooxygenase biology.organism_classification Molecular biology chemistry.chemical_compound Enzyme chemistry Pseudoplusia Insect Science parasitic diseases Botany medicine Noctuidae Acephate Permethrin medicine.drug |
| Zdroj: | Journal of Economic Entomology. 83:672-677 |
| ISSN: | 1938-291X 0022-0493 |
| DOI: | 10.1093/jee/83.3.672 |
| Popis: | Soybean looper, Pseudoplusia includens (Walker), larvae collected from a field where permethrin failed to provide adequate control and for which a reduction in susceptibility to permethrin had been demonstrated (3-fold at LC50) were compared with an established laboratory colony with respect to knockdown resistance ( kdr ) and in vitro metabolic capacity for a variety of substrates. The time necessary to achieve 50% knockdown of the field population (22.9 ± 1.3 min) after the topical application of 1 μg permethrin was significantly greater than that required for the laboratory colony (18.4 ± 1.0 min). Rates of metabolism for first-generation larvae from the field population were significantly greater than for larvae from the laboratory culture for substrates of glutathione transferase (l-chloro- 2,4-dinitrobenzene, 2.7-fold), monooxygenases (p-nitroanisole O-demethylase, l.8-fold), and hydrolases ( alpha -naphthyl acetate [1.5-fold], p-nitrophenyl acetate [1.5-fold], and permethrin [1.5-fold]). Significant differences between populations were not observed for NADPH cytochrome c reductase nor acephate hydrolysis. Results of the study indicate that a combination of target site insensitivity and increased activity of several enzymes involved in insecticide metabolism including a trans -permethrin hydrolase may be contributing to the reduced susceptibility of the field population relative to the laboratory colony. |
| Databáze: | OpenAIRE |
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