| Autor: |
B L Deyoe, Marshall Phillips |
| Rok vydání: |
1981 |
| Předmět: |
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| Zdroj: |
Veterinary Microbiology. 6:95-106 |
| ISSN: |
0378-1135 |
| DOI: |
10.1016/0378-1135(81)90002-x |
| Popis: |
Ribosomes from Brucella abortus strain 19 were isolated by differential centrifugation. The 70S ribosomes were purified by pelleting through 0.5 M ammonium chloride and 30% sucrose. The resulting ribosomes were designated washed ribosomes and consisted of 64–65% RNA and 35–36% protein with sedimentation coefficients of 50S and 30S. When the washed ribosomes were tested by immunodiffusion against immune bovine sera, no precipitins were detectable. The immunogenic responses of the washed ribosomes were determined in guinea pigs, lemmings, and calves. Inoculation dosages as low as 1.0 microgram in guinea pigs and 0.1 microgram in lemmings protected against challenge with virulent B. abortus . Results of similar studies in a limited number of calves indicated that a dose of 1 mg may be protective. Brucella ribosomes did not appear to elicit marked undesirable side effects when used as immunizing agents. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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