Comparative evaluation of the mesophilic and thermophilic biohydrogen production at optimized conditions using tequila vinasses as substrate

Autor: Alma Toledo-Cervantes, Jorge Arreola-Vargas, Francisco Villafán-Carranza, Hugo Oscar Méndez-Acosta, Elías Razo-Flores
Rok vydání: 2020
Předmět:
Zdroj: International Journal of Hydrogen Energy. 45:11000-11010
ISSN: 0360-3199
DOI: 10.1016/j.ijhydene.2020.02.051
Popis: The objective of this work was to comparatively evaluate the production of biohydrogen (bio-H2) from tequila vinasses at optimized mesophilic and thermophilic conditions and to elucidate the main metabolic routes involved. Optimal temperatures of 35 °C and 55 °C, and pH of 5.5 maximized the bio-H2 production rates, 25.5 ± 0.01 NmL h−1 and 169.9 ± 8.9 NmL h−1 in the mesophilic and thermophilic regimens, respectively. During the operation of anaerobic sequencing batch reactors, the thermophilic process allowed a volumetric bio-H2 production rate of 519 ± 13 NmL-H2 L−1 d−1 equivalent to 750 ± 19 NmL-H2 Lvinasse−1, while the mesophilic one 448 ± 23 NmL-H2 L−1 d−1 and 647 ± 33 NmL-H2 Lvinasse−1, respectively. Furthermore, the gas produced under thermophilic conditions showed high hydrogen content (86.5%). Finally, formate degradation and glucose fermentation to acetic and butyric acids were the main metabolic routes involved in bio-H2 production under thermophilic conditions, while at mesophilic conditions, the lactate and formate degradation pathways governed.
Databáze: OpenAIRE