Characterization of new microsatellite loci inPieris amamioshimensis(Ericaceae), a species nearly extinct in the wild
| Autor: | Junichi Nagasawa, Yuki Mitsui, Hiroaki Setoguchi, Yoshiyuki Maeda |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Genetics education.field_of_study Genetic diversity Ecology Population Locus (genetics) Plant Science Biology 010603 evolutionary biology 01 natural sciences Loss of heterozygosity Critically endangered Genetic marker Evolutionary biology Microsatellite Polymorphic Microsatellite Marker education Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Plant Species Biology. 33:72-76 |
| ISSN: | 0913-557X |
| DOI: | 10.1111/1442-1984.12193 |
| Popis: | A set of 11 polymorphic microsatellite markers has been developed and characterized for the critically endangered species Pieris amamioshimensis. Fifty-nine individuals of an ex-situ population were used to identify these markers. The total number of alleles for each locus ranged from 3 to 9, with an average of 5.4. The expected heterozygosities (HS) and observed heterozygosities (HO) ranged from 0.47 to 0.77 and 0.22 to 0.88, respectively. In total, four loci exhibited significant deviations from Hardy–Weinberg equilibrium: two loci showed significant heterozygosity excess and the other two loci showed significant heterozygosity deficit. The polymorphism information content (0.43 ≤ PIC ≤ 0.73), the probability of exclusions (PE1 = 0.9565, PE2 = 0.9969 and PE3 = 0.9999) and probabilities for identity (PI = 3.78 × 10−9 and PI-Sib = 2.35 × 10−4) suggest that these markers are useful for estimating not only genetic diversity but also parentage, for the ex-situ conservation management of populations. |
| Databáze: | OpenAIRE |
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