An investigation of the acyl-binding site of Candida antarctica lipase B
| Autor: | Richard W. McCabe, Alan Taylor |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Specificity constant
Adipic acid biology Stereochemistry Triacylglycerol lipase Active site Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound chemistry Acyl binding biology.protein Candida antarctica Binding site Lipase Biotechnology |
| Zdroj: | Enzyme and Microbial Technology. 35:393-398 |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/j.enzmictec.2004.04.019 |
| Popis: | The preference of the lipase Candida antarctica lipase B for dicarboxylic acids of a specific chain length has been demonstrated. The C6 adipic acid was found to be the preferred substrate as measured by the specificity constant for the esterification reaction. The effect of substitution on the substrate has been investigated and the carbonyl of 2-oxoadipic acid shown to stall the esterification reaction most probably by unfavourable hydrogen bonding within the active site of the enzyme. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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