| Popis: |
Over 50% of all human proteins are post-translationally modified by glycans, which alter their functions in fundamental biological processes. Mice (mus musculus) represent the most common mammalian model organism to study fundamental biological processes, including glycosylation. Because of isomeric variance, the comprehensive analysis of protein-linked N-glycans is a challenging task. Coupled with high-resolution mass-spectrometry, porous graphitic carbon (PGC)-LC is a powerful and widely used method for isomer-specific glycan analysis. Here we report on a consistent and complete PGC-LC-MS/MS N-glycome dataset of 23 different mouse tissues, critically complementing existing glycan data-repositories. We used PGC-LC to chromatographically separate even closely related N-glycan isomers and an Orbitrap Exploris 480 mass-spectrometer for data-acquisition. Multivariate data analysis revealed tissue specific N-glycome signatures, highlighted organ-intrinsic regulations of glycobiological pathways and confirmed prior glycobiological knowledge, as exemplified by the brain N-glycome. This dataset can be used for fundamental glycobiological research, glycan analytical benchmarking, the development of new mass-spectrometric data analysis tools, glycobiological pathway modelling and simulation, as well as for integrative systems biology. |
