Effects of M680I and M694V pyrin mutations on the tertiary structure of domain B30.2 and its interaction with caspase-1: In silico analysis

Autor:	Grigor Arakelov, Karen Nazaryan, O. V. Osipov
Rok vydání:	2015
Předmět:	Genetics In silico Biophysics Familial Mediterranean fever Biology medicine.disease Pyrin domain Protein tertiary structure Human genetics Domain (software engineering) Protein–protein interaction Structural Biology medicine Binding site
Zdroj:	Molecular Biology. 49:736-741
ISSN:	1608-3245 0026-8933
DOI:	10.1134/s0026893315050027
Popis:	The M680I and M694V mutations located in the B30.2 pyrin domain are responsible for the manifestation of the most common forms of Familial Mediterranean fever. It is well known that a malfunction of the pyrin–caspase-1 complex is the main cause of inflammation in FMF. The purpose of this study was to identify possible changes in the tertiary structure of mutated B30.2 domain and to determine their potential consequences in the formation of the pyrin–caspase-1 complex. Using computer modeling, it was found that the above mutations change the tertiary structure of B30.2 domain, causing shifts of binding sites and altering the energy of interaction between B30.2 and caspase-1.
Databáze:	OpenAIRE
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