Surface plasmon resonance sensing of a biomarker of Alzheimer disease in an intensity measurement mode with a bimetallic chip
| Autor: | Dae-ho Jang, Chang-Duk Kim, H.J.Kim, Young-Soo Sohn |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
business.industry Calibration curve 010401 analytical chemistry General Physics and Astronomy Nanotechnology 02 engineering and technology Substrate (electronics) 021001 nanoscience & nanotechnology Chip 01 natural sciences 0104 chemical sciences Intensity (physics) Laser linewidth Linear relationship Optoelectronics Surface plasmon resonance 0210 nano-technology business Bimetallic strip |
| Zdroj: | Journal of the Korean Physical Society. 69:793-797 |
| ISSN: | 1976-8524 0374-4884 |
| Popis: | A surface plasmon resonance (SPR) sensor system with a bimetallic chip has been utilized to sense the very low concentration of amyloid-beta (Aβ)(1-42) by measurement of the reflectance variation. The bimetallic chip was comprised of Au (10 nm) and Ag (40 nm) on Cr (2 nm)-coated BK-7 glass substrate. Protein A was used to efficiently immobilize the antibody of Aβ(1-42) on the surface of the bimetallic chip. The reflectance curve of the bimetallic chip represented a narrower linewidth compared to that of the conventional gold (Au) chip. The SPR sensor using the bimetallic chip in the intensity interrogation mode acquired the response of Aβ(1-42) at concentrations of 250, 500, 750 and 1,000 pg/ml. The calibration plot showed a linear relationship between the mean reflectance variation and the Aβ(1-42) concentration. The results proved that the SPR sensor system with the bimetallic chip in the intensity interrogation mode can successfully detect various concentrations of Aβ(1-42), including critical concentration, to help diagnose Alzheimer’s disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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