Human Embryonic Liver Cell Differentiation

Autor:	Volker E. Dube, Anwar A. Hakim, Charles M. Siraki
Rok vydání:	1986
Předmět:	Albumin Cell Biology General Medicine Biology medicine.disease Molecular biology Phenotype Embryonic stem cell digestive system diseases Pathology and Forensic Medicine Liver cell differentiation Cell culture Hepatocellular carcinoma medicine Cancer research Alpha-fetoprotein Molecular Biology
Zdroj:	Pathobiology. 54:319-332
ISSN:	1423-0291 1015-2008
DOI:	10.1159/000163371
Popis:	When incubated in preconditioned medium, i.e. spent media from the hepatocellular carcinoma (PLC/PRF/5) cell cultures, human embryonic liver (HEL) cells differentiate and acquire oncologic phenotypes. This is caused by transcriptional alterations in fetal gene expression. This occurs when hepatocytes are proliferating rapidly and secreting alpha fetoprotein (AFP), and later when the quiescent state is reached with secretion of albumin (ALB). The present studies examined the parameters signaling oncologic transformation during liver cell differentiation in the conditioned medium. mRNAs coding for AFP, ALB and HBsAg were isolated from HEL, adult liver cells (ADLC) and from PLC/PRF/5 cells, respectively. cDNA molecules complementary to these polysomal mRNA molecules were constructed and labeled with 32P. These tracers were used to quantitate changes in cellular mRNA • AFP, mRNA • ALB and mRNA·HBsAg directly by DNA molecular hybridization during HEL cells cultivation in the preconditioned medium. Under these conditions, the changes in cellular mRNA·HBsAg and mRNA • AFP correlated with an increased tumorigenicity in athymic Nu/Nu mice, membrane galactosyltransferase and phospho-tyrosine kinase activities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::06d79f5e8258f5d437791d04d0932d6a https://doi.org/10.1159/000163371 Zobrazit plný text záznamu