Autor: |
Chikage Aoki, Yutaka Aoki, Takashi Shimada, Atsuhiko Toyama, Taka-Aki Sato, Koichi Tanaka |
Rok vydání: |
2011 |
Předmět: |
|
Zdroj: |
Rapid Communications in Mass Spectrometry. 25:3521-3526 |
ISSN: |
0951-4198 |
Popis: |
One-step detection of biological molecules is one of the principal techniques for clinical diagnosis, and the potential of mass spectrometry for biomarker detection has been a promising new approach in the field of medical sciences. We demonstrate here a new and high-sensitivity method that we termed immunobeads-mass spectrometry (iMS), which combines conventional immunoprecipitation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The key feature of iMS is the MS-compatible condition of immunoprecipitation using detergents with a monosaccaride-C8 alkyl chain or a disaccharide-C10 alkyl chain, and the minimized number of steps required for high-sensitivity detection of target peptides in serum or biological fluid. This was achieved by optimizing the wash buffer and subjecting the immunobeads directly to MALDI-TOF MS analysis. Using this method, we showed that 1 fmol of amyloid beta peptide spiked in serum was readily detectable, demonstrating the powerful tool of iMS as a biomarker detection method. |
Databáze: |
OpenAIRE |
Externí odkaz: |
|