Virulence-associated genes and drug susceptibility patterns of uropathogenic Escherichia coli isolated from patients with urinary tract infection
| Autor: | Hamed Goodarzi, Nabi Jomehzadeh, Sajad Aslani, Mohamad Reza Akhond, Mohamad Hashemzadeh, Mina Moradzadeh, Samireh Azarpira, Mohammad Jaafar Yadyad, Mansoor Amin, Ahmad Farajzadah Sheikh, Reza Ranjbar |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pharmacology Phylogenetic tree Toxin 030106 microbiology Virulence Drug resistance Biology medicine.disease_cause Antimicrobial Microbiology Bacterial adhesin 03 medical and health sciences 0302 clinical medicine Infectious Diseases medicine Pharmacology (medical) 030212 general & internal medicine Escherichia coli Gene |
| Zdroj: | Infection and Drug Resistance. 12:2039-2047 |
| ISSN: | 1178-6973 |
| DOI: | 10.2147/idr.s199764 |
| Popis: | Background: Different Escherichia coli phylogenetic groups, such as A, B1, B2, and D, have four functional groups - adhesins, microcins, toxins, and capsules - which can cause urinary tract infections (UTIs). A phylogenetic group with a high virulence content becomes a worldwide health concern. Resistance to antimicrobial agents increasingly complicates the management of E. coli extraintestinal infections, as a major source of illness, death, and increased health care costs. The aim of this study was to determine the virulence content and the antimicrobial susceptibility pattern of different uropathogenic E. coli (UPEC) phylogenetic groups in Ahvaz, Iran. Methods: Phylogenetic groups, virulence-associated genes (VAGs), and antimicrobial susceptibility tests were detected by molecular and phenotypic methods in a total of 232 clinically well-characterized E. coli strains, isolated from two collections of patients with hospital-acquired (HA) and community-acquired (CA) UTIs. Results: Our results revealed that among 232 UPEC strains, the most frequent phylogenetic group was phylogroup D (58%) with the greatest content in virulence factors, including kpsM (23%), neuA (76.3%, capsule), cnf (29.6%, toxin), and Pap (54.8%, adhesin). Phylogroups D and, to a lesser extent, B2 were the most drug-resistant phylogroups. In addition, phylogroup D was responsible for the majority of HA (64.7%) and CA (48.4%) infections. Conclusion: Among UPEC strains causing UTIs, different phylogroups, through different VAGs, could cause severe infection. Knowledge about the distribution of the four functional groups and VAGs belonging to these phylogroups would significantly help to confine and prevent the development of lethal infection caused by these strains. |
| Databáze: | OpenAIRE |
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