Formation of protodioscin and deltoside isomers in suspension cultures of Nepal yam (Dioscorea deltoidea Wall.) cells
| Autor: | M. T. Khandy, Alexander Nosov, Maria Titova, Dmitry V. Kochkin, I. M. Ivanov, S. V. Konstantinova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Strain (chemistry) Cell growth Protodioscin Glycoside 030105 genetics & heredity Biology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry In vitro 03 medical and health sciences chemistry.chemical_compound chemistry Botany Bioreactor Composition (visual arts) Dioscorea Food science 010606 plant biology & botany |
| Zdroj: | Applied Biochemistry and Microbiology. 52:657-662 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683816060077 |
| Popis: | Changes in the content of the furostanol glycosides protodioscin and deltoside, particularly that of the (25S)-isomers of the glycosides, during suspension cultivation of different lines of Nepal yam (Dioscorea deltoidea Wall.) cells of the strain IFR-DM-0.5 has been investigated. The composition of furostanol glycosides has been characterized, and the dynamics of the accumulation of individual glycosides during lengthy subcultivation of cells maintained in flasks or in a barbotage bioreactor has been analyzed. A positive correlation between the growth and accumulation of substances that belonged to the class of furostanol glycosides has been demonstrated for cultured dioscorea cells, whereas the content of some of the individual glycosides varied considerably between the lines of the strain, cultures maintained under different conditions, and even between cells in different phases of the growth cycle. The increased content of (25R)-forms of the glycosides (protodioscin and deltoside) was correlated with a decrease in the cellular growth rate, whereas an increase in culture growth intensity occurred concomitantly to an increase of the amount of (25S)-isomers. This may be indicative of the specific stimulatory effect of (25S)-glycosides, but not the (25R)-forms, on cell proliferation in vitro. Thus, the concentration of (25S)-forms may increase due to the autoselection of cells capable of intensive division during prolonged cultivation. |
| Databáze: | OpenAIRE |
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