Advances in Gene Silencing Technology
| Autor: | Sitao Lin, Danqi Zhao |
|---|---|
| Rok vydání: | 2023 |
| Zdroj: | Theoretical and Natural Science. 4:554-558 |
| ISSN: | 2753-8826 2753-8818 |
| Popis: | Gene silencing is an important means of regulating gene expression in an organism, and this technique can reduce or block the expression level of a target gene. There are many types of gene silencing techniques, among which the more widely used are CRISPR/Cas9, TALEN, RNAi and so on. In recent years, with the gradual improvement and popularization of biotechnology, gene silencing techniques are slowly becoming known and applied in many fields such as gene research, disease treatment and breeding of new plant varieties. However, not all gene silencing can achieve the desired effect, and there are many reasons affecting the outcome of gene silencing. The target gene is not the only gene that determines the phenotype and there is no significant effect after knocking out the target gene. The technique also has a certain off-target rate, which can also lead to operational failure. This paper describes the principles and applications of three gene silencing techniques and compares the advantages and disadvantages of the three gene silencing techniques in order to select the most suitable gene silencing method from multiple perspectives. The current problems of CRISPR/Cas9 technology are summarized to provide certain ideas for the future development and research of CRISPR/Cas9 technology |
| Databáze: | OpenAIRE |
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