The Effect of Deuterium on Induction of the ada-Regulon with Alkylating Compounds in the Cells of Escherichia coli
| Autor: | S. K. Abilev, E. V. Igonina, N. K. Yankovsky, V. M. Glaser, V. N. Parmon, S. V. Smirnova |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0301 basic medicine Promoter N-Nitroso-N-methylurea Alkylation Biology medicine.disease_cause Methyl methanesulfonate 03 medical and health sciences DNA Alkylation chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Regulon Biochemistry chemistry Transcription (biology) 030220 oncology & carcinogenesis Genetics medicine bacteria lipids (amino acids peptides and proteins) Escherichia coli |
| Zdroj: | Russian Journal of Genetics. 54:919-924 |
| ISSN: | 1608-3369 1022-7954 |
| DOI: | 10.1134/s1022795418080124 |
| Popis: | This study has shown for the first time that deuterium oxide (D2O) enhances expression of the E. coli ada-regulon induced by alkylating compounds, N-nitroso-N-methylurea (NMU) and methyl methanesulfonate (MMS). To compare the induction of ada-regulon in deuterated and in non-deuterated (control) cultures of E. coli, a biosensor based on the E. coli strain K12 MG1655 (pAlkA–lux) luminescing as a result of activating the alkA gene promoter in response to DNA alkylation was used. Depending on the D2O concentration from 5 to 10% in the pre-deuterium medium, these alkylating compounds at a concentration of 0.005 M induced expression of the alkA gene from 1.3 to 5 times higher as compared to induction in nondeuterated culture. It is suggested that deuterium can be one of the main factors in stabilizing the bond between the promoter and the alkylated Ada protein, leading to increased transcription of the ada-regulon. |
| Databáze: | OpenAIRE |
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