Influence of Five-Element Music Therapy on Expression of CaMKII in Hippocampi in Rats with Post-Stroke Depression
| Autor: | Facai Lin, Yong Bian, Feng Hao, Yujing Pan, Yunchuan Wu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Fluoxetine Music therapy 030504 nursing business.industry Traditional Chinese medicine humanities 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Concomitant Ca2+/calmodulin-dependent protein kinase Acupuncture Medicine Post-stroke depression Hippocampus (mythology) 0305 other medical science business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | 2018 9th International Conference on Information Technology in Medicine and Education (ITME). |
| DOI: | 10.1109/itme.2018.00052 |
| Popis: | Objective: To explore the influence of traditional Chinese medicine (TCM) five-element music therapy on the expression of type II Ca2+/calmodulin-dependent protein kinase (CaMK-II) in calmodulin signal pathway in cerebral hippocampus tissues in rats with post-stroke depression (PSD), so as to explain the action mechanism of five-element music therapy in the treatment of PSD. Methods: A total of 40 male SD rats with body mass of (220 ± 30) g were selected as study subjects and randomized into normal group, PSD model group, music therapy group, music therapy + acupuncture group and Fluoxetine group, 8 rats in each group. After one week of adaptive feed, PSD models were made. The treatment started on post-molding d19. Music therapy group and music therapy + acupuncture group were treated for 7 days as one cycle, with 2 days of rest between treatment cycles, for consecutive 2 cycles, while Fluoxetine group was treated with continuous gavage since start of treatment, qd, until the end of treatment. Hippocampus tissues were collected from rats’ brains, after which quantitative polymerase chain reaction (qPCR) was used to detect the expression of CaMKⅡgene, while western blot (WB) to detect the expression of CaMKⅡprotein in rats’ hippocampi. Results: Relative to normal group, model group was evidently lower in expression of CaMKⅡgene and protein (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|