Autor: Eduard Tararak, O. P. Il'inskaya, E. V. Gracheva, N. N. Samovilova, N. K. Golovanova, N. V. Prokazova
Rok vydání: 2001
Předmět:
Zdroj: Biochemistry (Moscow). 66:397-401
ISSN: 0006-2979
DOI: 10.1023/a:1010245328209
Popis: Sialyltransferase activity has been determined in Golgi membrane fractions isolated from atherosclerotic and normal intima of human aorta by measuring the transfer of N-acetylneuraminic acid (NeuAc) from CMP-NeuAc to asialofetuin. The asialofetuin-sialyltransferase activity was found to be twofold higher in the atherosclerotic intima than in the normal intima. The mean value of the apparent Michaelis constant (Km) for the sialylating enzyme in both tissues did not differ and was 57 μM. In contrast, the maximal velocity (Vmax) was 2-fold higher for the atherosclerotic intima than for the normal intima. These results suggest that expression of asialofetuin-sialyltransferases of the aortal intima may be increased in atherosclerosis.
Databáze: OpenAIRE
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