Infectious abortion caused by Salmonella enterica subsp enterica serovar Abortusequi in Argentina

Autor: A.J. Muñoz, P.G. Franco, Nora Guida, E.E. Di Gennaro, E.V. Moras
Rok vydání: 2012
Předmět:
Zdroj: Journal of Equine Veterinary Science. 32:S74
ISSN: 0737-0806
DOI: 10.1016/j.jevs.2012.08.158
Popis: The equine host-adapted serovar, Salmonella enterica subsp enterica (See) serovar Abortusequi (SeeA) cause a disease called equine paratyphoid. The disease is manifested as abortion with other signs, including fistulous withers, septicemia, and arthritis. SeeA is transmitted by the ingestion of contaminated food with uterine secretions of aborted mares [1]. Different episodes of reproductive failure have recently been reported at several farms in the Province of Buenos Aires. Some of these episodes were studied at the Laboratory-School of Diagnosis of Infectious Diseases at the Veterinary University, and in two of these cases, the isolation of the SeeA bacterium was posible. The recovery of this bacterium gives an alarm signal; therefore, we should reconsider former agents responsible for reproductive diseases which now re-emerge due to the lack or discontinuation of preventive immunizations or due to critical points that we should not dismiss when considering the epidemiology of the agent. The objective of this study is to identify the cause of abortions that showed clinical, anatomic and pathological features of the bacterial abortions reported at different farms. Placentas from aborted mares and aborted fetuses were examined. For the isolation of bacteria, heart blood, liver, spleen, lung, intestine and stomach content were cultured in pre enrichment and selective enrichment medium. The enrichment medium was streaked onto XLD and MacConkey agar plates. Salmonella like isolates were identified by biochemical analysis using primary and secondary test as described Cowan and Steel, 2003. [2] The serotype was verified at the Bacteriology Departament, INEI ANLIS "Dr. Carlos G. Malbran. Dra. Maria Ines Caffer, Three placentas from aborted mares and three aborted fetuses were examined. Fibrinonecrotic exudates or congestion were observed at the placentas. Liver and lung congestions and intestine petechial hemorrhages were observed at the fetuses. See was isolated from two fetuses from heart blood, liver, spleen and lung. UBA 874 Salmonella Abortusequi (4,12:-:e,n,x) and UBA 879 Salmonella Abortusequi (4,12:-:e,n,x) were identified from the two spleens. The strains were used for developing formalin-inactivated bacterins in Tymerosal and OHAl adjuvant. The SeeA is a hard-to-isolate bacterium so in this study it was isolated in two samples only. Both isolated samples were enough to conclude that we are facing a reemerging disease. It is known that animals with positive serologic response as well as vaccinated animals cannot be reinfected by the Salmonella bacterium. The complete aborted animal, fetal organs, placenta or even fecal material are adequate samples for cultivation since isolation and identifications provide the definitive diagnosis.
Databáze: OpenAIRE
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