Magnetic interactions and in vitro study of biocompatible hydrocaffeic acid-stabilized Fe–Pt clusters as MRI contrast agents
| Autor: | Samo Hudoklin, Zvonko Jagličić, Janez Ščančar, Nina Kostevšek, Janja Vidmar, Igor Serša, Ana Sepe, K. Žužek Rožman, Sašo Šturm, Mateja Erdani Kreft, Spomenka Kobe |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Biocompatibility
Chemistry General Chemical Engineering MRI contrast agent Nanoparticle Infrared spectroscopy 02 engineering and technology General Chemistry engineering.material 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Coating engineering In vitro study Fourier transform infrared spectroscopy 0210 nano-technology Cytotoxicity Nuclear chemistry |
| Zdroj: | RSC Advances. 8:14694-14704 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c8ra00047f |
| Popis: | A detailed magnetic study of separated Fe–Pt NPs and Fe–Pt clusters was performed to predict their optimal size and morphology for the maximum saturation magnetization, a factor that is known to influence the performance of a magnetic-resonance-imaging (MRI) contrast agent. Excellent stability and biocompatibility of the nanoparticle suspension was achieved using a novel coating based on hydrocaffeic acid (HCA), which was confirmed with a detailed Fourier-transform infrared spectroscopy (FTIR) study. An in vitro study on a human-bladder papillary urothelial neoplasm RT4 cell line confirmed that HCA-Fe–Pt nanoparticles showed no cytotoxicity, even at a very high concentration (550 μg Fe–Pt per mL), with no delayed cytotoxic effect being detected. This indicates that the HCA coating provides excellent biocompatibility of the nanoparticles, which is a prerequisite for the material to be used as a safe contrast agent for MRI. The cellular uptake and internalization mechanism were studied using ICP-MS and TEM analyses. Furthermore, it was shown that even a very low concentration of Fe–Pt nanoparticles ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|