Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily
| Autor: | R. J. Ridge, John Coughlin, D Banach, Hermann Oppermann, C. Corbett, R M Whetstone, T. K. Sampath, D. C. Rueger, Engin Ozkaynak |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Molecular mass Protein subunit Proteolytic enzymes Transforming growth factor beta superfamily Biological activity Cell Biology Biology Biochemistry Molecular biology Amino acid chemistry.chemical_compound chemistry Sodium dodecyl sulfate Glycoprotein Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 265:13198-13205 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(19)38285-7 |
| Popis: | A bone-inductive protein has been purified from bovine bone and designated as osteogenic protein (OP). The purified OP induces new bone at less than 5 ng with half-maximal bone differentiation activity at about 20 ng/25 mg of matrix implant in a subcutaneous bone induction assay. The purified osteogenic protein is composed of disulfide-linked dimers that migrate on sodium dodecyl sulfate gels as a diffuse band with an apparent molecular weight of 30,000. Upon reduction, the dimers yield two subunits that migrate with molecular weights of 18,000 and 16,000. Both subunits are glycosylated. After chemical or enzymatic deglycosylation, the dimers migrate as a diffuse 27-kDa band that upon reduction yields two polypeptides that migrate at 16 kDa and 14 kDa, respectively. The carbohydrate moiety does not appear to be essential for biological activity since the deglycosylated proteins are capable of inducing bone formation in vivo. Amino acid sequences of peptides generated by proteolytic digestion show that the subunits are distinct but related members of the transforming growth factor-beta super-family. The 18-kDa subunit is the protein product of the bovine equivalent of the human OP-1 gene and the 16-kDa subunit is the protein product of the bovine equivalent of the human BMP-2A gene. |
| Databáze: | OpenAIRE |
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