Improved Method for Obtaining of Arctigenin from Arctium Lappa L. and its Antiproliferative Effect on Human Hepatocarcinoma HepG2 Cells
| Autor: | Valquíria P. Medeiros, Paulo Henrique Dias de Carvalho, Renan S. Teixeira, Ademar A. da Silva Filho, Jorge Willian Leandro Nascimento, Jair A.K. Aguiar |
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| Rok vydání: | 2020 |
| Předmět: |
Traditional medicine
Chemistry Improved method 01 natural sciences 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 030220 oncology & carcinogenesis Hepg2 cells Arctium lappa General Pharmacology Toxicology and Pharmaceutics Antiproliferative effect Arctigenin |
| Zdroj: | Current Bioactive Compounds. 16:358-362 |
| ISSN: | 1573-4072 |
| Popis: | Background: Arctigenin is a lignan found in Arctium lappa L. (Asteraceae) that displays anti-inflammatory activities. Previous studies showed that the crude extract of A. Lappa has antitumor activity in human liver carcinoma, lung and stomach cancer cells. The aim of this study was to obtain arctigenin from A. lappa L., as well as to evaluate its antiproliferative effects in cells of liver carcinoma (HepG2) and fibroblasts (NIH/3T3). Methods: Arctigenin was obtained from the hydrolysis of arctiin, which was isolated from the crude extract of A. lappa. The effects of arctigenin and arctiin on HepG2 cell viability and cell adhesion were analyzed by MTT method. Adhesion assay was also carried out to evaluate the antitumor activity. Results: Our results showed that the analytical process to obtain arctigenin was fast and easy. In vitro experiments showed that arctigenin (107-269 μM) decreased HepG2 cells viability and did not cause cytotoxicity on NIH/3T3 cells. Arctigenin (27-269 μM) demonstrated anti-adhesion in HepG2 cells in a concentration-dependent manner, when compared with control. Conclusion: These results suggest a promising pharmacological activity for arctigenin as an antiproliferative compound. |
| Databáze: | OpenAIRE |
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