| Autor: |
Willén, Katarina, Edgar, James, Hasegawa, Takafumi, Tanaka, Nobuyuki, Futter, Clare, Gouras, Gunnar |
| Rok vydání: |
2017 |
| Předmět: |
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| DOI: |
10.6084/m9.figshare.c.3861745_d5.v1 |
| Popis: |
ESCRT proteins in primary neurons and plaques. (A-B) In young 3-month-old Tg19959 mice, CHMP2B immunolabelling is increased in areas of hippocampus (a) and entorhinal cortex (b) that also have increased labelling of APP/Aβ (6E10). Scale bar 40 μm, n = 4. (C) Western blot analysis of APP/PS1 compared to wt primary neurons at 12 DIV lysed in 6% SDS shows that protein levels of CHMP2B and VPS4 are not significantly changed, although there is a trend for increased levels of CHMP2B in APP/PS1 neurons, n > 6. Protein levels are expressed as percentage of control and are corrected against actin. (D) VPS4 colocalizes with Aβ42 in a vesicular pattern in 19-month-old wt mice (upper panel), n = 3. In 19-month-old APP/PS1 mice VPS4 accumulates in and around amyloid plaques (lower panel, white arrows). Scale bar 40 μm, n = 4. (E) Decreased labelling of CHMP2B in plaques (white arrows) in 19-month-old APP/PS1 mice. Some colocalization of CHMP2B is seen in Aβ/APP (6E10) positive cells (grey arrows). Scale bar 40 μm, n = 3. (F) Labelling of early ESCRT-0 component Hrs is decreased in amyloid plaques compared to surrounding brain parenchyma. Scale bar 40 μm, n = 2. (PDF 269 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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