Mass-Spectrometric MRM Analysis of FDA-Approved Proteins in Plasma of Healthy Volunteers

Autor: V. G. Zgoda, N. A. Shushkova, Alexander I. Archakov, S. E. Novikova, M. A. Pyatnitsky, E. A. Ponomarenko, T. E. Farafonova, A V Lisitsa, A. I. Grigoryev, Victor A. Tutelyan, O. V. Tikhonova
Rok vydání: 2021
Předmět:
Zdroj: Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry. 15:40-61
ISSN: 1990-7516
1990-7508
DOI: 10.1134/s1990750821010054
Popis: The proteomic composition of a biological sample serves as the most important feature of a biological object, and it allows discriminating normal and pathological conditions. Targeted mass spectrometric analysis, particularly, multiple reaction monitoring (MRM) using synthetic stable isotope-labeled internal standards (SIS), is the main alternative to the ELISA method for analysis of diagnostically significant proteins. Based on the MRM results, a prototype test system has been developed; it employs the targeted mass spectrometric method for multiplex, quantitative analysis of FDA-approved proteins in whole plasma. Using this approach, it was possible to measure the content of 42 proteins in 31 samples in a concentration range spanning five orders of magnitude. The interindividual variability for 30 of the 42 registered proteins was less than 40%. The largest scatter was observed for haptoglobin (68%), immunoglobulin heavy constant delta IGHD (90%), angiotensin (72%), sex hormone-binding globulin SHBG (100%), and lipoprotein-(a) (136%). The obtained results on the concentration of proteins correlate with published data (Hortin et al., Clinical Chemistry, 2008, vol. 54, 1608) with R2 = 0.84. The developed prototype test system based on targeted mass spectrometric analysis of proteins can be considered as an alternative to methods using monoclonal antibodies.
Databáze: OpenAIRE
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