Combined antibacterial activities of essential oil compounds against Escherichia coli O157:H7 and their application potential on fresh-cut lettuce

Autor:	Wenqian Yuan, Hyun-Gyun Yuk, Constance Hui Min Teo
Rok vydání:	2019
Předmět:	Chemistry Vanillin Antimicrobial Citral law.invention Eugenol chemistry.chemical_compound Linalool law Carvacrol Food science Thymol Essential oil Food Science Biotechnology
Zdroj:	Food Control. 96:112-118
ISSN:	0956-7135
DOI:	10.1016/j.foodcont.2018.09.005
Popis:	This study evaluated the antimicrobial activities of combined use of essential oil (EO) compounds against E. coli O157:H7, using the Fractional Inhibitory Concentration (FIC) index and time-kill assay. The mechanisms of enhanced antimicrobial efficacies in EO combinations were evaluated using flow cytometry, and their application potentials as decontamination rinse were assessed using fresh-cut lettuce. Thymol (Thy), carvacrol (Car), trans-cinnamaldehyde (TC), eugenol (Eug) and vanillin (Van) displayed minimum inhibitory concentrations (MICs) in a range of 0.63–2.5 mg/mL, whereas citral (Cit) and linalool (Lin) were ineffective (MIC > 10 mg/mL). FIC indices (1.00–2.00) suggested additive interactions of all EO combinations, and time kill assay showed enhanced bactericidal activities in Thy/Eug, Car/Eug and TC/Eug combinations. The stronger antimicrobial efficacy in EO combination was attributed to increased membrane damages. Application of Thy/Eug and Car/Eug on fresh-cut lettuce as decontamination rinse caused a significant (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0600ff9fb32187ba31a0ff1501808bf1 https://doi.org/10.1016/j.foodcont.2018.09.005 Zobrazit plný text záznamu Full Text from ScienceDirect