Development and validation of a high-performance liquid chromatographic method for the analysis of antioxidative phenolic compounds in fennel using a narrow bore reversed phase C18 column
| Autor: | Irene Parejo, Francesc Viladomat, Carles Codina, Jaume Bastida |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Foeniculum biology Rosmarinic acid Extraction (chemistry) Reversed-phase chromatography biology.organism_classification Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Environmental Chemistry Sample preparation Phenols Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 512:271-280 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2004.02.053 |
| Popis: | A simple high-performance liquid chromatography (HPLC) method for the separation and quantitative determination of the main antioxidant phenolic compounds from bitter fennel, Foeniculum vulgare , was developed. The use of a narrow bore reversed phase (RP) C 18 column and an acidic mobile phase enabled ten compounds (caffeoylquinic acids, dicaffeoylquinic acids, flavonoids and rosmarinic acid) to be separated within a 40 min time analysis. The method was validated to demonstrate its selectivity, linearity, precision, accuracy and robustness. In addition, some parameters were studied to optimize the complete extraction of the phenolic compounds. The method was applied to the evaluation of three different fennel materials: distilled and non-distilled aerial parts, as well as defatted fruits. Distilled fennel was found to contain a higher proportion of antioxidant phenolic compounds than the non-distilled plan material. |
| Databáze: | OpenAIRE |
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