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We studied the regulatory effects of noradrenaline (NA) on antibody and cytokine production in cultured SAMP1 spleen cells stimulated with ovalbumin (OVA), a T-dependent antigen. SAMP1 and C3H/He mice were immunized with an intraperitoneal injection of OVA with alum adjuvant. Spleen cells primed to OVA as well as unprimed naive spleen cells were then cultured with OVA in the presence of varying concentrations of NA. The supernatant from each culture was assayed for interferon (IFN)-γ, interleukin (IL)-4, and anti-OVA antibodies by enzyme-linked immunosorbent assay (ELISA). OVA-primed SAMP1 spleen cells produced as much IFN-γ as C3H/He spleen cells. At the concentrations tested, NA did not augment but rather suppressed the IFN-γ production of both SAMP1 and C3H/He at concentrations higher than 3.0×10 −5 M. Furthermore, the OVA-primed SAMP1 spleen cells tended to show lower IL-4 production than the C3H/He cells. NA at 3.0 ×10 −6 M augmented the IL-4 production of OVA-primed C3H/He cells, but not SAMP1 spleen cells. NA at concentrations higher than 3.0×10 −5 M suppressed the IL-4 production of both SAMP1 and C3H/He spleen cells. These results suggest that the machinery to produce Th2-type cytokines in SAMP1 mice has an abnormal response to the regulatory signal of NA. |
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