Sugarcane glycoproteins are required to the production of an active UDP-glucose dehydrogenase byXanthomonas albilineans
| Autor: | Carlos Vicente, María Blanch, Dolores Piñón, María-Estrella Legaz |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Proteases Protease biology Chloramphenicol medicine.medical_treatment Dehydrogenase biology.organism_classification Applied Microbiology and Biotechnology Microbiology Enzyme chemistry Biochemistry Xanthomonas albilineans medicine Glycoprotein Bacteria medicine.drug |
| Zdroj: | Annals of Microbiology. 57:217-221 |
| ISSN: | 1869-2044 1590-4261 |
| DOI: | 10.1007/bf03175210 |
| Popis: | In vitro enzymatic assay of UDP-glucose dehydrogenase fromXanthomonas albilineans requires the addition of a protease inhibitors cocktail to cell-free extracts, since bacterial proteases rapidly hydrolyses the enzyme in solution. The addition of low amounts of 8-azaguanine and chloramphenicol to the culture medium do not impede the production of the dehydrogenase that requires concentrations higher than 0.3 mM of both antimetabolites to inhibit its synthesis. Glycoproteins from sugarcane, the natural host of the bacterium, also assure the production of the active enzyme by inhibiting bacterial proteases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink