Gut inflammation exacerbates hepatic injury in C57BL/6J mice via gut-vascular barrier dysfunction with high-fat-incorporated meat protein diets
| Autor: | Muhammad Umair Ijaz, Chunbao Li, Syed Umar Farooq Bukhary, Muhammad Ijaz Ahmad, Muzahir Hussain, Waqar Ali Khan, Majid Suhail Hashmi, Iftikhar Ali Khan, Sayed Hussain |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Gut flora 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Fibrosis Lipid droplet Internal medicine medicine Oil Red O Liver injury biology business.industry Binding protein General Medicine medicine.disease biology.organism_classification 030104 developmental biology Endocrinology chemistry Lipogenesis 030211 gastroenterology & hepatology business Food Science |
| Zdroj: | Food & Function. 11:9168-9176 |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/d0fo02153a |
| Popis: | Aim: Meat and its derivatives provide nutrients essential for human health. However, meat consumption, along with excessive fat intake, has been associated with gut inflammation, intestinal barrier dysfunction and alterations in gut microbiota. Herein, we investigated whether and how these changes in the intestinal barrier system affect the gut liver axis and hepatic injury and eventually lead to the progression of liver syndrome such as NAFLD. Methods: Mice were fed with high fat (60% kcal) or low fat (12% kcal) along with soybean (control), chicken and pork proteins (HFCH, HFP, LFCH, and LFP) for 12 weeks. The biomarkers for liver injury were investigated after meat protein intake along with the high fat. Findings: Greater amount of fat vacuoles visible in the H&E staining increased the inflammatory cell infiltration and disorganized liver structures were observed in the HFP-fed mice. Oil Red O staining revealed that the HFP-fed and HFCH-fed mice showed more lipid droplets, confirming the increased hepatic lipid accumulation. Potential serum markers for NAFLD, ALT and AST were increased in the HF meat diet groups. Key genes responsible for hepatic inflammation and lipogenesis, such as MCP-1, IL1-β and TNF-α were upregulated. HF meat protein diet-fed mice exhibited signs of compromised liver with increased levels of endotoxin in the liver and its binding protein in serum, upregulation of TLRs in the liver, and significant increase in TG, TC, LDL-C and HDL-C concentrations. Significance: Intestinal inflammation and barrier dysfunction aggravate liver injury and fibrosis due to the intake of HF meat protein diets in mice, which may contribute to the progress of liver injury and associated complications. Gut inflammation may directly contribute to the development of NAFLD, especially of the gut vascular barricade dysfunction. |
| Databáze: | OpenAIRE |
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