A triazole‐based fluorescence probe for detecting Hg 2+ ions and its biological application
| Autor: | Liu Rongqiang, Wen Ying He, Guo Hua Ding, Jian Ling Li, Lu Yong Wu, Hua Jie Feng |
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| Rok vydání: | 2019 |
| Předmět: |
Electrospray
Chemistry Metal ions in aqueous solution 010401 analytical chemistry Biophysics 02 engineering and technology Carbon-13 NMR 021001 nanoscience & nanotechnology Photochemistry 01 natural sciences Fluorescence 0104 chemical sciences Rhodamine chemistry.chemical_compound Chemistry (miscellaneous) Rhodamine B Moiety 0210 nano-technology Spectroscopy |
| Zdroj: | Luminescence. 35:129-137 |
| ISSN: | 1522-7243 1522-7235 |
| DOI: | 10.1002/bio.3705 |
| Popis: | A new compound, ethyl 5-phenyl-2-(p-tolyl)-2H-1,2,3-triazole-4-carboxylate was successfully introduced and synthesized as a novel rhodamine B derivative named REPPC, and characterized by 1 H nuclear magnetic resonance (NMR), 13 C NMR, and high resolution mass spectrometry (HRMS). It showed an obvious fluorescence and UV-visible light absorption enhancement towards Hg2+ ion without interference from common metal ions in N,N-dimethylformamide-H2 O (pH 7.4). The spirolactam ring moiety of rhodamine in REPPC was converted to the open-ring form generating a 1:1 complex with the intervention of a mercury ion, verified by electrospray ionization-mass spectroscopy testing and density functional theory calculation. REPPC was used to visualize the level of mercury ions in living HeLa cells with encouraging results. |
| Databáze: | OpenAIRE |
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