Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images
| Autor: | Jean Ollion, Thomas Boudier, Christophe Escudé, Julien Cochennec, François Loll |
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| Rok vydání: | 2014 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Nuclear gene business.industry Process (engineering) Computer science Nuclear organization computer.software_genre Genome Nuclear architecture Cell nucleus Software medicine.anatomical_structure Quantitative analysis (finance) Organelle medicine Fluorescence microscope Data mining business Throughput (business) computer |
| Zdroj: | The Nucleus ISBN: 9781493916795 |
| Popis: | The cell nucleus is a highly organized cellular organelle that contains the genome. An important step to understand the relationships between genome positioning and genome functions is to extract quantitative data from three-dimensional (3D) fluorescence imaging. However, such approaches are limited by the requirement for processing and analyzing large sets of images. Here we present a practical approach using TANGO (Tools for Analysis of Nuclear Genome Organization), an image analysis tool dedicated to the study of nuclear architecture. TANGO is a generic tool able to process large sets of images, allowing quantitative study of nuclear organization. In this chapter a practical description of the software is drawn in order to give an overview of its different concepts and functionalities. This description is illustrated with a precise example that can be performed step-by-step on experimental data provided on the website http://biophysique.mnhn.fr/tango/HomePage. |
| Databáze: | OpenAIRE |
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