A distinct RNA recognition mechanism governs Np 4 decapping by RppH
| Autor: | Rose Levenson-Palmer, Daniel J. Luciano, Nikita Vasilyev, Ashok Nuthanakanti, Alexander Serganov, Joel G. Belasco |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences. 119 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.2117318119 |
| Popis: | Significance Dinucleoside tetraphosphate alarmones function in bacteria as precursors to 5′-terminal nucleoside tetraphosphate (Np 4 ) caps, becoming incorporated at high levels into RNA during stress and thereby influencing transcript lifetimes. However, little is known about how these noncanonical caps are removed as a prelude to RNA degradation. Here, we report that the RNA pyrophosphohydrolase RppH assumes a leading role in decapping those transcripts under conditions of disulfide stress and that it recognizes Np 4 -capped 5′ ends by an unexpected mechanism, generating a triphosphorylated RNA intermediate that must undergo further deprotection by RppH to trigger degradation. These findings help to explain the uneven distribution of Np 4 caps on bacterial transcripts and have important implications for how gene expression is reprogrammed in response to stress. |
| Databáze: | OpenAIRE |
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