Isolation and Characterization of Methanolobus bombayensis sp. nov., a Methylotrophic Methanogen That Requires High Concentrations of Divalent Cations

Autor:	Dilip R. Ranade, Linda Mandelco, Priya C. Kadam, David R. Boone
Rok vydání:	1994
Předmět:	chemistry.chemical_classification biology Immunology Methanolobus biology.organism_classification Microbiology Methanogen Divalent Cell wall chemistry Biochemistry Yeast extract Methanomicrobiales Bacteria Methanosarcinaceae
Zdroj:	International Journal of Systematic Bacteriology. 44:603-607
ISSN:	1465-2102 0020-7713
DOI:	10.1099/00207713-44-4-603
Popis:	Methanolobus bombayensis B-1T (= OCM 438T) (T = type strain) was isolated from Arabian Sea sediments obtained near Bombay, India. This strain grew on methylamines, methanol, and dimethyl sulfide, but it did not catabolize H2-CO2, acetate, or formate. The cells were nonmotile, irregular coccoids (diameter, 1.0 to 1.5 μm) and occurred singly. Electron micrographs revealed that a cell membrane and a protein cell wall were present. The cells grew fastest at mesophilic temperatures, at a neutral pH, and at salinity levels near the salinity level of the ocean, and they required about 30 mM divalent cations (Mg2+ and Ca2+). The cells grew in mineral medium, but growth was greatly stimulated by yeast extract and peptones. The guanine-plus-cytosine content of the DNA was 39.2 ± 0.1 mol%. A comparison of 16S rRNA sequences showed that strain B-1T was phylogenetically related to Methanolobus vulcani, but the sequences of these organisms differed by 2%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0572bf5f4171850a4851ad8c1c2259e5 https://doi.org/10.1099/00207713-44-4-603 Zobrazit plný text záznamu