An increase in the resistance of R-phycoerythrin to thermal aggregation by silver nanoparticles synthesized in nanochannels of the pigment
| Autor: | Boris I. Kurganov, O. D. Bekasova, Vita Stein-Margolina, V. V. Shubin, L. I. Kovalyov, Vera A. Borzova |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Circular dichroism Materials science Hydrodynamic radius 030102 biochemistry & molecular biology Analytical chemistry Nanoparticle Photosynthetic pigment Applied Microbiology and Biotechnology Biochemistry Silver nanoparticle law.invention 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Chemical engineering Dynamic light scattering law Thermal stability Electron microscope |
| Zdroj: | Applied Biochemistry and Microbiology. 52:98-104 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683816010026 |
| Popis: | R-phycoerithrin, a water soluble photosynthetic pigment of red algae, was used as a matrix and a reducer for the synthesis of Ag0 nanoparticles. The diameter of dominating Ag0 nanoparticles determined from the electron microscope images was 6.5 ± 0.5 nm. Dynamic light scattering in the regime of heating with a constant rate showed that the hydrodynamic radius of R-phycoerithrin molecules began to increase at a temperature of more than 50°C. The hydrodynamic radius of R-phycoerithrin with Ag0 nanoparticles synthesized in the inner cavity remained constant upon heating to 90°C. It was shown by circular dichroism that the temperature at which the portion of the denatured protein reached 50% was 51.7 ± 0.1°C for R-phycoerithrin and 58.0 ± 0.1°C for R-phycoerithrin with synthesized Ag0 nanoparticles. This indicated that thermal stability of R-phycoerythrin increased when its cavity was filled with an Ag0 nanoparticle. According to the comparative spectral studies of R-phycoerythrin before and after the synthesis of Ag0 nanoparticles and the heat treatment, we conclude that an increase in thermal stability of R-phycoerythrin is caused by chemical intramolecular cross-linking of a protein molecule with an Ag0 nanoparticle. |
| Databáze: | OpenAIRE |
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